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TSA-1.2


Super-Agarose®

TSA-1.2:  $11.89 †

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Size-select and concentrate your DNA or RNA right in your Gel! Recovering intact nucleic acids from a gel is simplified with our special Super-Agarose® formulation. Once you have run your gel, cut a small trough in front of your band(s) of interest, fill it in with our ready made Super-Agarose, and electrophorese in the nucleic acid.

     The Super-Agarose formulation decreases the DNA's electrophoretic mobility to near zero; thus messy broad bands, and even multiple bands, pile-up and are compressed into a minimum volume of agarose (fig. 1). That means you get all of your nucleic acid in a few microliters of high-quality, low-melting temperature gel, rather than in a few hundred microliters. Plus there is no loss or shearing of high molecular weight molecules since they remain in the gel.

     Use Super-Agarose to obtain preparative amounts of DNA or RNA from minor bands, or of size-selected genomic DNA (fig. 2). Run your gel as usual, loading several adjacent lanes with the same sample of interest; cut out the appropriate band across those lanes; cut another perpendicular hole, slightly longer than your band slice, elsewhere in the gel (or in a new gel). Place your band slice in the new hole and fill the extra space (toward the anode) with Super-Agarose (fig.2). Your nucleic acid will run until it becomes highly concentrated within our formulation: ready for your demanding application! (fig.2) Virtually all enzymatic reactions can be performed in gelo or you can extract if you wish.

Every batch of Super-Agarose® is tested and guaranteed to be free of nuclease, protease, and enzyme inhibitors.


† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.
 

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