The Pipectrode® represents a major technical advance to electroporation. The most important factors for highest transformation efficiency are low toxicity, temperature control of the sample during electroporation and rapid recovery afterward. Thin surgical stainless steel electrodes backed by water-ice temperature stabilizers provide a superior environment for cells during electroporation. We make use of the "latent heat of fusion" of water (it takes the same number of calories to melt 1g of ice as to heat 1g of water 80°C!), so your sample stays cool, which is important to avoid arcing. Since the Pipectrode is a pipette, you can eject your sample into recovery buffer instantly. Comparison Costs for 100 Transformations (>2 x 109/µg)** | Company | "Cuvettes" | Cells* | Supply Total | Time in batches of 5 | | BioRad | 2 x $135 | 10 x $99 | $1260 | 200 min. | | BRL | 3 x $70 | 10 x $150 | $1710 | 300 min. | | BTX | 2 x $126 | none available | $1800? | 200 min. | | Invitrogen | 2 x $125 | 10 x $175 | $1900 | 200 min. | | Stratagene | 2 x $135 | 10 x $135 | $1620 | 200 min. | | Strategene | "Ultra Comp." | 10 x $145 | $1450 | 700 min. | | and a world apart... | | Cloning Gun | 2 x $75 | 5 x $50 | $400 | <17 min. |
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