The Cloning Gun™ is a cordless, rechargeable, hand-held electroporation instrument that achieves transfection efficiencies exceeding 50% of viable cells with a variety of standard mammalian cell lines, and Transformation Efficiencies (TE's) of over 1010 transformants per µg of input DNA with E. coli bacteria. The completely portable, hand-held design makes transformation a one-step process. Just plug in one of our revolutionary disposable Pipectrode™ pipetting electrode tips to The Cloning Gun. Draw your cell/DNA mixture into the Pipectrode using the ergonomic precision thumbwheel-operated pump, pull the trigger to zap it, and eject your transformants into a flask or Petri dish - all in less than 15 seconds! Unlike other systems, our Pipectrode makes the time consuming process of transferring your cell/DNA mixture back and forth with cuvettes and pipettes obsolete. When you compare the low cost of The Cloning Gun and its Pipectrode chambers with the cost of conventional electroporators and cuvettes, chemically competent cells, or lipofection reagents, the increased efficiency in terms of transformants per dollar is quite impressive. If you figure in time and convenience, especially in high-throughput experiments, the increased efficiency is truly remarkable. Finally, electroporation is a simple low-cost procedure for every scientist. Don't pay more for the antiquated technology offered by other manufacturers. You can use The Cloning Gun right at your own bench, or for contamination-sensitive samples, take it to the sterile hood. The MammoZapper™ version of The Cloning Gun™ is a unique non-ionic, minimally-conductive, near isotonic electroporation medium and ultra-short pulse time increases both transfection efficiency and post-zap viability. We took the guess-work out of electrical parameters by doing the electroporation research here. The Cloning Gun voltage and time parameters have been optimized to give excellent results with mammalian cells including neurons, astrocytes, neuroglia, lymphocytes, monocytes, fibroblasts, epithelial and endothelial cells from human, mouse, rat and monkey (see photos). Just ask us if we have tried it on your favorite cells, or get a gun and see for yourself (there is a 30-day money-back guarantee on all Tritech Research products). The thumbwheel pump and Pipectrode™ electroporation chambers are so much more user friendly than cuvettes and transfer pipettes that we guarantee The Cloning Gun is the most efficient and convenient electroporator you will ever have the pleasure of discharging.
The BactoZapper™ version of The Cloning Gun™ revolutionizes E. coli transformation. The Cloning Gun comes factory pre-set to the optimal parameters for transformation. TE can be greater than 1010 per µg of input DNA for cells harvested in phase. Even with standard stationary phase cells, prepared using our revolutionary, super-easy cell prep featuring our CompBooster™ Solution, overnight cultures can exceed 109per µg. Key Cloning Gun Features:- Pipectrodes cost less than cuvettes, yield a higher TE (due to reduced cytotoxicity of the thin stainless steel electrodes as compared with ionizing aluminum electrodes), yet are more environmentally friendly.
- While sold as "disposable", Pipectrodes can be reused many more times than cumbersome cuvettes.
- You don't need an oscilloscope to know that it is working - simple red, yellow, and green indicator lights let you know if your sample and DNA performed according to normal parameters.
- At least ten times faster than any other electroporation device.
- Ergonomic design.
- Small and portable, cordless/rechargeable.
- Use it at your own bench or in the sterile hood!
BactoZapper™ Features:- Much less expensive per transformation than commercially available competent cells.
- 100-fold higher transformation efficiency, with much faster sample prep as compared with home-made CaCl2 cells.
- Only The Cloning Gun™ is optimized to work with the revolutionary Stationary Phase cell prep, developed at Tritech Research, which can yield over 2 x 109 transformants per µg using simple overnight cultures.
MammoZapper™ Features:- Unlike other methods, electroporation only introduces your molecule of interest (not extra calcium, detergents, lipids, projectiles, or viral particles).
- Extremely high transfection efficiency without complicated expensive viral- mediated protocols.
- More reliable and generally applicable than CaPO4 co-precipitation.
- Does not require costly lipid-based products.
The Bar-Chart below shows transformation results with various cell types. Click on the graph bars to see actual pictures of the transformed cells.  For a recent comparison of the Cloning Gun to other electroporators by The Scientist Magazine, click here for a copy of the article on our site, or here for The Scientist Website.
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