Narishige's IM-11-2 is a pneumatically operated microinjector that allows suction and injection of a microsample with precision and operability comparable to oil hydraulic injectors. It features maintenance-free operation for IVF and transgenic work. If you were considering an Eppendorf Celltram or a "mushroom" injector, consider the IM-11-2 as an alternative for both quality features and price.
This model incorporates a new pneumatic injection mechanism, with extremely smooth control and a reduced dead volume for superior responsiveness comparable to oil-filled microinjectors. Since it doesn't use oil, there are no maintenance issues like topping up the oil, re-filling, or removing air bubbles. New quick-connect fittings allows for quickly changing tubing and needle holders without tools! Coaxial course and fine control knobs enable pressure to be changed both quickly and with high precision. This injector is recommended for use for both injecting on the microinjection side and also for the cell holding side. The unit is self-contained (no additional syringes needed).
There are two identical versions of this product available. The IM-11-2 is "For Research Use Only" and the IM-11-2a is an identical product that is FDA registered for medical procedures such as IVF / ICSI. The IM-11-2a has an extra fee associated with it to cover the Medical Device Excise Tax that is charged as per the PPACA ("Obamacare"). Please specify which model number you want at the time of order.
A variation of the IM-11-2, designated IM-12, is a shorter/stubbier version of the IM-11-2 with only course control. If you need the IM-12, we can special-order it from Narishige Japan - contact us for a quote.
Key Features
1) Quick-connect tubing connection The quick-connect fitting makes it easy to insert, remove, or replace the tubing that goes to the pipette holder, using just your fingers. The same fitting is found on the included HI-9 needle holder, so it is now trivial to replace the tubing or remove the needle holder for cleaning. The quick-connects also allow the tubing to be rotated so that it doesn't kink, thus the needle tip can be rotated by rotating the holder.
2) Pressure release push-button A one-touch pressure relief valve allows pressure to vent from the injector so you can zero the pressure at any point within the travel of the internal syringe. This makes it easy to adjust the responsiveness, create the perfect balance pressure in the pipette, or instantly release an egg or stop an injection.
3) Responsiveness is adjustable As mentioned in feature 2, by zeroing the pressure at larger or smaller syringe volumes, the operational responsiveness can be decreased or increased, respectively, according to your preference.
4) Course and fine pressure adjustment A long syringe and coarse/fine coaxial control knobs allow the amount of pressure change per knob rotation to be increased by approx. 25x compared to other injectors with only a fine movement. This gives access to a wide range of pressures, quickly, without sacrificing fine pressure control.
Accessories for IM-11-2 are made exclusively for IM-11-2. Please contact us before considering other accessories.
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These are our list prices. If you are paying with an Institutional
Purchase Order or by check, you qualify for a 7.5% discount. Click
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Precision Hydraulic Microinjector (oil-type)
IM-21:
$2,270.27
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Narishige's IM-21 is a compact, oil microinjector with new functions that simplify the oil-filling process. It can be used for various purposes such as ICSI, injection of DNA, RNA, CRISPR Cas9 in mammalian eggs and fish eggs, injection of ES cells and IPS cells, nuclear transfer and cell pick-up. This unit is the highly-modernized replacement for IM-9A, IM-9B, IM-6, IM-6-2 and CellTram® oil
Multiple Functions
Changeable Plunger Movement: Plunger can be moved back and forth without turning control knob. Provides easy oil filling and air bubble removal.
Built-in Channel Switch: No need to connect 3-way stopcock. Allows you to switch the flow channel easily.
Easy Tube Connection: Insert tubing and tighten knob.
Built-in Plastic Cylinder: Provides clear visibility through plastic.
A video about filling the IM-21 with oil
To watch the video larger, Click the full-screen arrows. To watch in a separate window with captions, Click on "Watch on Youtube"
Specification
Accessories included
[page IM-H3]IM-H3 Injection Holder Set* O Ring Silicone Rubber Gasket Silicone Grease Cleaning Rod
Movement range
Plunger 20mm Full rotation of knob 500um
Control capacity
Full rotation of knob approx. 10ul
Dimensions/Weight
W127 - 147 x D56 x H78mm, 620g
Tube Length
1m
CONSUMABLES HI01PK01 - Silicone Rubber Gasket, 2.5mm x 1.0mm ID (10pcs). *[page IM-H3]IM-H3 is a set consisting of [page HI-7]HI-7, [page CT-1]CT-1.
†
These are our list prices. If you are paying with an Institutional
Purchase Order or by check, you qualify for a 7.5% discount. Click
here to change your payment method.
Syringe-based Microinjector
IM-3:
$832.43
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- Special Order
Narishige's IM-3 is a compact and simple injector and is used with a microsyringe, such as the Hamilton. The syringes are prepared separately.
Specification
Acceptable syringe
5mm - 10mm
Movement range
Fine
X40mm Full rotation of knob 500um
Dimensions/Weight
W130 x D23 x H85mm, 230g
*This is a Narishige "Group-2" special order item. Because it has to be custom-made for you, there is usually a 4-week lead time and it is non-cancellable and non-returnable once ordered. Prices for Group-2 items change often, and, like all of our website prices are subject to change without notice, so always double-check with us prior to placing an order for this item.
†
These are our list prices. If you are paying with an Institutional
Purchase Order or by check, you qualify for a 7.5% discount. Click
here to change your payment method.
Microinjector for performing injections into the brain
IMS-4:
$1,754.59
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This is a manual injector designed for brain injection which is used with a glass syringe. Attached to the stereotaxic micromanipulator SMM-series, turning the control knob by hand pushes the plunger slowly, allowing injection of small volume. Suction is also possible as the plunger is fixed by a knob and pulled. The syringe is attached and detached from the front of the main body, which permits handling a syringe without hitting the tip of a sterilized needle. The connecting bar is included in the accessories for the stereotaxic micromanipulator, and the mounting bracket can change the orientation of setup. Suitable for large-volume injection and ICV administration, etc.
Specification
Acceptable syringe
ø5 - ø10mm
Movement range
Fine 50mm Full rotation of knob: 500µm
Dimensions/Weight
W45 × D52 × H143mm, 251g
†
These are our list prices. If you are paying with an Institutional
Purchase Order or by check, you qualify for a 7.5% discount. Click
here to change your payment method.
Tritech Microinjectors
microINJECTOR™ All-Digital Positive Displacement System
For applications that require a specific volume to be delivered or aspirated in the nanoliter / microliter / milliliter range, at a defined rate, Tritech Research offers the motorized MINJ-PD Digital Positive Displacement microINJECTOR™. Unlike most other microinjection controllers, this system does not require attachment to a gas cylinder, pump, or compressed air line. It works like an electronic syringe pump and is completely self-contained.
With the injection volume and speed set in advance, this motor-driven microinjector injects very small volumes of liquid with precision control via a foot switch, leaving both hands free for micromanipulation and focusing the microscope. In addition to fixed injection volumes, there is also a Manual Mode that allows the user to inject without knowing the volume in advance. In Manual Mode, the MINJ-PD displays a tally of the amount injected.
The MINJ-PD was originally designed for stem cell injection into blastocysts, and is now used for stem cell injections into human spinal cords. We're proud to say that (while For Research Use Only) it is currently the only FDA-listed device for injecting neural cells into the spinal cords of ALS patients and people with spinal cord injuries by the scientists at Neuralstem, Inc. and their collaborators worldwide. This injector is also used for micro-perfusion and delivery of drugs, neurotransmitters, and vectors to brain, retina, and cultured cells in neurobiology, for injecting insects in pathogen studies, and for many other applications. In 2017, we revised the hardware and firmware to support smoother, more precise delivery, easier loading of sterile syringes, and to add a Pressure Mode.
In addition to the original Positive-Displacement Mode, where the MINJ-PD syringe, tubing, etc. are filled with liquid for hydraulic action, a new Pressure Mode allows the MINJ-PD to be partially or completely air-filled for pneumatic injection control without the need of an external pressure source! In this mode, when you push the foot pedal, the syringe advances to create a positive pressure that makes things flow from the needle; when you release the pedal, the syringe returns to its neutral position to stop the injection flow. Inversely, the unit can be used to aspirate.
There are distinct advantages to Positive-Displacement Mode vs. Pressure Mode. In Positive-Displacement Mode, it is possible to set a specific delivery volume and delivery rate. Unfortunately, this is only practical for volumes over 1 µL because thermal expansion, elasticity, etc., become significant compared with volumes smaller than this. Pressure Mode can deliver volumes arbitrarily small, for example in the picoliter range, but the actual volume will be determined by the pressure, time, needle hole size, and viscosity of the the substance.
For stem cell injection, a special built-in Agitation Mode helps to prevent cells from clumping and settling on the wall of the needle between injections.
The MINJ-PD employs the same user-friendly microprocessor interface as the MINJ-D. It digitally synthesizes injecting / aspirating volume and speed, as well as agitating volume and speed, reproducibly. Using a simple "Menu" button, and Plus and Minus buttons, anyone can set the volumes and the speeds in seconds. Here are the key features and specifications of the Positive Displacement Digital microINJECTOR:
Most compact and robust on the market
6" x 7" x 3" and weighs under 2 lbs.
(15cm x 17cm x 7cm and weighs under 1 kg)
Simple 3-button control
Digital display with backlight for easy reading
Microprocessor accuracy
Foot pedal included for hands-free injection control
Total syringe volume: 100uL (standard) ([page MINJ-PD-S100]MINJ-PD-S100), or available 250 uL ([page MINJ-PD-S250]MINJ-PD-S250), and 500uL ([page MINJ-PD-S500]MINJ-PD-S500) options
Programmable Injection volume: 0.01 - 50.0uL (with 100 uL syringe)
Injection volume up to 400 uL with 500 uL syringe)
Programmable Injection speed: 0.2 - 250.0s/uL
Programmable Agitation volume: 0.1 - 25.0uL
Programmable Agitation speed: 0.2 - 25.0s/uL
Manual Injection Mode
New "Pressure Mode"
New syringe holder for quick change of pre-loaded and/orsterile syringes
Optional Bi-directional Computer Interface for full automation
Optional TTL trigger for automated injection
The output of the microINJECTOR needs to be coupled to something. For example, if you're using glass microcapillary needles, our [page MINJ-4]MINJ-4 microINJECTOR™ Brass Straight-Arm Needle Holder will do the job cost-effectively. Please note that, for use with the MINJ-PD, a special version of all MINJ-4 variations will be supplied that uses a gas-tight swage fitting and terminates in a Luer/Slip fitting to attach conveniently to the MINJ-PD's syringe. The pictures below show the MINJ-4 variations with a quick-connect fitting that looks a bit different than what you will receive.
[page MINJ-4]
An autoclavable option with similar capabilities to the MINJ-4 is Narishige's [page IM-H1]IM-H1. [page IM-H1]
A special version, the [page MINJ-4FEM] MINJ-4FEM, is designed for those that prefer to use Eppendorf® prepulled Femtotips® (that screw on) instead of our own lower-cost [page MINJ-PP]MINJ-PP prepulled needles that are compatible with our standard [page MINJ-4] MINJ-4. [page MINJ-4FEM]
Another special version, the [page MINJ4-SLIP]MINJ-4SLIP, is designed for those who prefer to use SlipTip® and Luer-Lok® Needles (that push or twist on) instead of our [page MINJ-PP]MINJ-PP glass needles. [page MINJ-4SLIP]
Here are videos that shows how to use all the functions of and to load the MINJ-PD:
Here is a video that shows how to set up the MF2 for first use (it is similar to the MF-900 shown):
To watch the video larger, Click the full-screen arrows. To watch in a separate window with captions, Click on "Watch on Youtube"
†
These are our list prices. If you are paying with an Institutional
Purchase Order or by check, you qualify for a 7.5% discount. Click
here to change your payment method.
