Tritech Research sells complete microinjection systems that integrate our famous microINJECTOR™, Pulse Length Control Module (PCM), and needle holder with top name brand inverted microscopes and micromanipulation equipment. We take advantage of our status as a fully authorized Narishige dealer and manufacturer of custom OEM solutions for Olympus and other microscope systems to get you great prices and the added convenience of receiving a fully integrated microinjection system from a single source.

Other features include our custom GlideStage®, which provides a fine level of manual stage manipulation at a fraction of the cost of motorized stage manipulators. When the stage is in use, you can employ a low-cost mechanical joystick-type micromanipulator, to save thousands of dollars over other systems.

Microscope Features:

Small Foot Print.
Excellent Quality Optics.
Includes our custom-made Hoffman Modulation Contrast (HMC) optics for DIC-like images at a significantly reduced price.
Trinocular head for photo and video documentation.
Available with a fluorescent system for detection of GFP, DAPI, Rhodamine, etc.

Some researchers are curious whether a low-cost dedicated injection station like the MINJ-1000 is going to be a compromise in terms of quality, ease-of-use, or optics. The answer is - no. Great optics are as important to us as they are to you. Just as there can be a "good" DIC image, when the right Wallaston prism is combined with the right numerical aperture objective and the correct polarization, and a poor DIC image when these items are not optimized, we design and choose our HMC optics to work together to get you great images. Don't let a bad experience with a local microscope rep who doesn't know what he is doing, or understand how to optimize a system for your microinjection needs prejudice you.

Here are links to some QuickTime movies showing oocytes and the syncitial gonads of some C. elegans worms under our MINJ-1000 scope. We think you will be impressed with how clear and easy to inject these structures are. Click on these links to watch the QuickTime Movies (it will take a while for them to load completely, and then they will play.) To watch one again, click "Reload" or hit the play button if it is visible:

gonad04.mov (6.2MB)
gonad05.mov (7.3MB)
gonad06.mov (4.0MB, lower res)

And, here are a few still images... Click them to see an enlarged view:

COMPARISON OF HOFFMAN AND DIC VIEWS
OF THE SAME C. ELEGANS WORM


HMC	DIC

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Microinjectors

Motor-Drive Microinjector (Programmable)

IM-300: $5,081.08 † - Ships in 1-5 days

Image for Motor-Drive Microinjector coming soon!

Narishige's IM-300 is a multi-functional, motor-driven injector for precise control of small volumes.

Setting the time and pressure in advance makes it possible to inject very small volumes of liquid with great precision. This model's numerous capabilities, including injection, filling up and holding, and varied functions such as foot switching, programming, and air distribution, make it suitable for a wide variety of applications. External pressure sources are used, and should be selected according to the experiment.

Specification

Accessories included	Input Hose, Output Hose, Foot Switch, Silicone Rubber Gasket, Power Supply Cable, HI-7 Injection Holder
Pressure	7kg/cm^2 maximum
Power cunsumption	Approx. 35W
Dimensions/Weight	W425 x D205 x H90mm, 3.7kg

*To Use this model, a compressor or compressed gas tank and regulator is required.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Supports various applications from microinjecting cells to puffing a drug solution to stimulate cells.

IM-31: $3,891.89 † - Ships in 1-5 days

Narishige's IM-31 is a versatile electric microinjector, ideal for labs performing a broad range of microinjections. It has 2 modes - a count mode to set the pressure / time / count and inject a solution and a foot switch mode to set only the pressure and inject a solution while pressing a foot switch - that can be selected in accordance with the experimental application. In addition, incremented settings are also possible when the count is set. Moreover, external control with the same action as the foot switch is possible via the input of TTL signals (negative logic).

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Pneumatic Injector

IM-5A: $1,027.03 † - Ships in 1-5 days

Narishige's IM-5A is a pneumatic microinjector ideally suited for use in placing on the the cell holding side and can control a large volume of liquid. The large, stable body makes operation extremely reliable. It can be operated pneumatically, avoiding maintenance issues like topping up the oil, re-filling or removing air bubbles.

*Oil top-up kit IM-AS (sold separately) is available for easy oil top-up and air bubble removal.

*For details of attaching a commercially available syringe to this model, please contact us.

Specification

Accessories included	IM-H1 Injection Holder Set(*1), Silicone Rubber Gasket, Glass Syringe 3ml, Polyethylene Tube
Movement range	Plunger 40mm, Full rotation of knob: approx. 6mm
Control capacity	Total volumes 3ml, Full rotation of knob approx. 480ul
Dimensions/Weight	W200 x D80 x H130mm, 2kg

(*1)IM-H1 is a jointed set consisting of HI-7, CT-1 and CI-1.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Pneumatic Injector

IM-9C: $1,189.19 † - Ships in 1-5 days

Narishige's IM-9C is a pneumatic microinjector with maintenance-free operation.

This model features the same new mechanism as the IM-9B, offering smooth control by reducing fluctuations of the handle tension. As well as controlling a large volume of liquid, it can be operated pneumatically, avoiding maintenance issues like topping up the oil, re-filling or removing air bubbles. Mainly recommended for use in placing the cell holding side.

*Dedicated large size syringe incorporated.

Specification

Accessories included	IM-H2 Injection Holder Set(*1), O Ring, Silicone Grease, Polyethylene Tube, Silicone Rubber Gasket
Movement range	Plunger 53mm, Full rotation of knob approx. 6mm
Control capacity	Total volumes 4240ul, Full rotation of knob approx. 480ul
Dimensions/Weight	W136- 189 x D55 x H74mm, 0.64kg

(*1)IM-H2 is a jointed set consisting of HI-7, CT-1 and CI-3.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Tritech Microinjectors

microINJECTOR™ System

MINJ-1: $643.24 † - In Stock

Image for microINJECTOR(TM) System coming soon!

Tritech Research has designed a microinjection system that lets you control with exacting precision both the pressure and the duration of the microinjection pulse. And, it's available at a fraction of the cost of competing systems.

Our microINJECTOR™ System uses a precision pressure regulator and a high-speed electronic solenoid switching valve, yet it is amazingly robust and simple to operate. It allows you to start and stop injection within a fraction of a second and control the quantity and flow of material into your target with precision. Our electronic foot control is designed to keep your hands free so you can concentrate on the manipulation of your needle and target.

The microINJECTOR comes preassembled, ready to use. You need only attach the system to a compressed gas tank (e.g. N₂) or in-house pressurized air line at one end and your needle at the other. You set the desired pressure and the system delivers pulses of pressure to your needle. In the base model, the duration of the injection pulse is controlled by how long you hold down the high-speed electronic foot pedal. With our easy-to-use, reliable equipment there is no "magic" required. Even a novice will be turning out transformants the first day.

With our optional electronic Pulse-Length Control Module (PCM) you can set injection times from 0.1 to 15 seconds. Each time the pedal is pressed, a constant volume is reproducibly injected. This option is crucial for quantitative experiments such as transient expression assays following microinjection of DNA, RNA, protein or other substances.

By controlling the pulse length and pressure independently, you can compensate for the flow characteristics of individual needles; you can determine if the needle is properly placed prior to any appreciable damage occurring to your target; and you can fill your target incrementally. High pressure settings with rapid on/off pulses aid in unclogging needles.

As an added option to the microINJECTOR System you can hold your target with our state-of-the art Holding Pipette Controller (HPC). The HPC allows you to pick-up and hold your target with vacuum suction and then release it with positive pressure. Our unique pneumatic foot pedal control allows a continuous range from vacuum through positive pressure depending on the position of the pedal. It is ideal for holding and manipulating targets such as embryos and oocytes. Also useful for injecting ES cells into Blastocysts. As any of our customers will attest, within a short time you will be proficiently getting transformants.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

microINJECTOR™ All-Digital Multi-pressure System

MINJ-D: $3,237.84 † - In Stock

For demanding applications that require a full-featured microinjectionsystem, Tritech Research offers the MINJ-D All-Digital Multi-Pressure microINJECTOR™. In basic microinjection, a hollow needle with a microscopic tip is filled with a liquid, poked into a target, and then temporarily pressurized so as to inject some of the liquid into the target. The variables that determine the amount injected are pressure, time, viscosity of the liquid, and diameter of the needle opening. A simple, practical system for accomplishing this is our MINJ-1 microINJECTOR™ (standard model). While the MINJ-1, and its line of accessories, help many scientists breeze through their microinjections, some experiments and procedures require a higher level of sophistication, accuracy, and reproducibility. In basic microinjection, the needle is either at a user-set pressure when injecting, or at atmospheric pressure when not injecting. In some cases, backflow of cytoplasm or medium into the needle can be problematic. For these cases, a lower "holding pressure" that is lower than the injecting pressure, but higher than atmospheric pressure is useful between injections. A holding pressure can also reduce needle clogging. No matter how careful a person is, needles occasionally do clog. For these cases, a short blast of a "clearing pressure," higher than the normal injection pressure, is useful to unclog the needles. The MINJ-D employs a user-friendly microprocessor to digitally synthesize injecting, holding and clearing pressures, as well as injection durations reproducibly. Using a simple "Menu" button, and Up and Down buttons, anyone can set the pressures and the injection duration in seconds. It's so intuitive, you won't have to read the directions! Here are the key features and specifications of the Digital microINJECTOR™:

Most compact on the market
6" x 7" x 3" and weighs under 2 lbs.
(15cm x 17cm x 7cm and weighs under 1 kg)
Fast response time between pressures (~100 milliseconds)
Simple 3-button control
Digital display with backlight for easy reading
Microprocessor accuracy
Foot pedal included for hands-free injection control
Pressure range (applies to each of holding, injecting and clearing pressures): 0 - 100 psi
Programmable Injection Duration: 0.1 - 999.0 seconds
Built-in Vacuum Generator can be used to front-fill needles by aspiration
Manual Injection Mode
Injection Data-logging Port for optional computer interface
Injection Control Port for optional computer control

The MINJ-D can be attached directly to a laboratory compressed air jet or attached to a compressed Nitrogen cylinder using a pressure regulator such as our TREG-N2. The system is designed to use a minimum of gas, so even a small cylinder may last for months or years. When ordering, please let us know if you intend to attach your injector to a compressed gas source or an in-house air jet, so that we can provide you with the proper fittings for attachment.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.