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Narishige's PC-10 micropipette puller is a dual-stage micropipette processing system which provides reliable and consistent production of micropipettes with tip sizes ranging from one micron to tenths of a micron.

The PC-10 incorporates several key features that make it an ideal choice for producing microinjection needles and pipettes for patch clamp experiments. The built-in power source is specially stabilized to deliver a uniform amount of current to the heating coil each time. The stabilized delivery of power ensures consistent and even heating of micropipettes time after time.

Heater levels for both single and double pull operations can be adjusted, and the heater level is shown by a highly-visible digital LED readout display.

During the pulling process, micropipettes are shielded from air currents by the use of an acrylic shroud. This excludes external drafts that might otherwise affect the shape and size of the micropipette.

Two pairs of weights (two light and two heavy) are provided for adjustment of the pulling force. Also included are two replacement heater coils (PC-10H), and 100 pieces of 1.0mm I.D. glass microcapillary tube with internal glass fiber (GD-1).

Narishige is using this more automatic and full-featured PC-10 to replace the old semi-automatic PP-830 that was discontinued in 2006. The PC-10 is also the replacement for the even older model PB-7 and it serves as a lower-cost more robust alternative to Sutter's P-30. In fact, we use the PC-10, here at Tritech Research, to pull our pre-pulled microcapillary needles (see MINJ-PP) that we sell for C. elegans, Drosophila, Xenopus, Zebrafish, and transgenic mouse work.

Specification

Accessories included	GD-1 Glass Capillary with filament, Spare Heater, Power Supply Cable
Power source	AC100V(+-5%), AC120V(+-5%), AC220V(+-5%), AC240V(+-5%)
Power consumption	Approx. 80W
Heater level	100 = heater voltage 2.5V
Weight	Approx. 25g x 2, Approx 100g x 2
Dimensions/Weight	W205 x D190 x H185mm, 6.0kg

Consumable

Model	Product	Description
PC-10H	Heater	Kanthal wire (x5)

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.

Glass Microelectrode Puller

PE-22: $8,054.05 † - Ships in 1-5 days

Narishige's PE-22 produces multibarreled electrodes, longer and thinner electrodes, and other special microelectrodes.

The PE-22 is an updated version of the previous PE-21 and PE-2 pullers, which were long established as the best Ecclecs and tower type pullers, which featured new electrical characteristics for greater safety and stability. For safer operation, the PE-22 has a metal cover attached to both the heater unit and the shaft.

Like conventional pullers, the PE-22 stretches the glass capillary vertically by using the gravitational force of its own weight and the weight of the magnet. The device that holds a glass capillary employs a drill chuck system that accepts 2 - 6mm glass capillaries. Moreover, using a bundle of 1mm glass capillaries, multibarreled microelectrodes can be produced.

From capillaries ø2 to ø4mm thick, the PE-22 can produce the long, comparatively firm electrodes used for nerve center experiments, or multibarreled electrodes combining three, four, and seven ø1mm glass capillaries. The magnet and heater are adjusted from the digital display where the maximum output of each is represented as 100: this makes adjustment easy and contributes to greater accuracy in mass production. By combining two conventionally separated units into one, the model saves a considerable amount of space.

Tritech Research recommends the PE-22 Needle Puller in nerve center experiments where microelectrodes are inserted deep into the brain. In such experiments, parallelism is essential to a long tapered tip. To achieve the parallelism required, the adjustable range of the microswitch has been increased. As compared to conventional models, the PE-22 is capable of producing microelectrodes of more different shapes. The microswitch is now calibrated and thus easy to adjust, which contributes to improved reproducibility.

Specifications

Accessories included	Glass Capillaries (2mm) Glass Capillaries (3mm) Spare Heater Chuck Handle Power Cord * PE-21HC (for 3mm) is fitted to the main unit.
Power source	AC100 (±5%), 50/60Hz AC120 (±5%), 50/60Hz AC220 (±5%), 50/60Hz AC240 (±5%), 50/60Hz
Power Consumption	Approx.500W at max.
Heater Current	Approx.17A
Heater Voltage	Approx.6V
Dimensions/Weight	W280 × D280 × H610mm, 20.7kg

Consumables

Model	Product	Description
PE-21HA	Straight heater for PE-2/21	Straight Kanthal wire (×5)
PE-21HB	Coil heater for PE-21	For 2mm Kanthal wire (×5)
PE-21HC	Coil heater for PE-21	For 3mm Kanthal wire (×5)
PE-21HD	Coil heater for PE-21	For 6mm Kanthal wire (×5)

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.