The 2009 Gordon Conference on Antimicrobial Peptides will present the latest research on the biology and activities of antimicrobial peptides, essential and multifunctional host defense molecules employed by all organisms for innate defense. The Conference will feature a wide range of topics, such as structure/function analysis, regulation of expression, mechanisms of action, microbial avoidance strategies, human disease associations and model systems, and development of antimicrobial peptides as therapeutics. Invited speakers represent a diverse variety of scientific disciplines, including biochemistry, molecular genetics, microbiology, animal and plant biology, structural biology, medicine, innate immunity, signaling, and drug development. The Conference will bring together a collection of investigators who are at the forefront of their field, and will provide opportunities for junior scientists and graduate students to present their work in poster format and exchange ideas with leaders in the field. Some abstracts and poster presenters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented.

The 2009 Gordon Conference on Evolutionary & Ecological Functional Genomics will present cutting-edge snapshots of ways in which genomics approaches are being used to study adaptation of organisms to changing environments, genome evolution and population genetics, as well as the role of genomic evolution in the evolution of complex phenotypes. In particular, several of the talks this year will highlight "global change genomics" and evidence for evolution at the molecular and phenotypic levels in response to climate change. A diversity of sessions will highlight recent results from natural as well as experimental populations, and will cover both non-model and model species. There will be an exciting balance between ecologists using genomics to study adaptation in rapidly evolving systems, as well as genomicists turning large-scale sequence, expression and functional assays to study variation among populations and species. Several sessions will emphasize recent findings on plant ecological genomics. Other topics will include the evolutionary genomics of infectious disease; microbial metagenomics; population genomics of model species (such as humans, Drosophila and yeast); and the genomics of speciation.

The conveners, Scott Edwards (Harvard) and Loretta Johnson (Kansas State University) have invited approximately 25 world-class speakers in these diverse areas, all of whom will be presenting at this particular Gordon conference for the very first time. Many are young investigators with exciting new systems to discuss whereas we also have several established leaders of the field. The conveners are committed to assembling a diverse conference and are actively applying for funds to allow graduate students and others to attend. Although attendance at Gordon conferences is by application, we intend to admit as many as we can on a first-come-first serve basis, and the limit for this particular conference is 165 attendees. We hope to see you there!

The Gordon Research Conference on Mycotoxins and Phycotoxins offers an international forum for the presentation of leading-edge research on fungal, algal, and cyanobacterial toxins and the organisms that produce them. This multidisciplinary gathering will encompass the biology, ecology, genetics, chemistry and the public health and economic impacts of these natural hazards. In 2009, the conference will convene at Colby-Sawyer College in New London, New Hampshire, June 21-26. The goals of the conference are to assemble a critical mass of leading-edge scientists and students, and to create an atmosphere for creative thinking to advance knowledge of the complex problems presented by these naturally occurring organisms and toxins. Considerable effort is made to provide opportunities for participants, outstanding scientists and aspiring students alike, to interact in relaxed surroundings that promote the free exchange of ideas on topics vital to the future of research on the societal and environmental impacts of mycotoxins and phycotoxins. The 2009 conference will have a particular emphasis on the influence of climate change on the biogeography of toxic algae, cyanobacteria and fungi; the genomics and proteomics of toxin production; the prediction and detection of hazardous events; and the public health and economic impacts of these natural hazards. The conference is unique in that it brings together a diverse multidisciplinary group of research scientists and students who are all ultimately focused on solving similar problems -- ensuring the health of the environment and the safety and security of the food supply.

The 2009 Gordon Conference on Atherosclerosis will present cutting-edge research on the molecular, cellular, immunological, structural and therapeutic aspects of atherosclerotic plaque formation and regression. The Conference will feature a wide range of topics, such as macrophage subsets, vascular calcification, vascular progenitor cells, genetic regulation of lipid metabolism, nanotechnology for diagnosis and treatment of atherosclerosis, cardiac valve atherosclerosis, inflammatory mediators, systems biology, lesion dynamics and reversibility, and clinical translation to novel therapeutics. Invited speakers represent a variety of scientific disciplines, including biochemistry, lipid metabolism, molecular genetics, bioengineering, immunology, vascular biology, cell biology and imaging. The Conference will bring together a collection of investigators who are at the forefront of their field, and will provide opportunities for junior scientists and graduate students to present their work in poster format and exchange ideas with leaders in the field. Some poster presenters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented.

The 2009 Gordon Conference on Microbial Population Biology will cover a diverse range of cutting edge issues in the microbial sciences and beyond. Firmly founded in evolutionary biology and with a strongly integrative approach, past Conferences have covered a range of topics from the dynamics and genetics of adaptation to the evolution of mutation rate, community ecology, evolutionary genomics, altruism, and epidemiology. The 2009 Conference is no exception, and will include sessions on the evolution of infectious diseases, social evolution, the evolution of symbioses, experimental evolution, adaptive landscapes, community dynamics, and the evolution of protein structure and function. While genomic approaches continue to make inroads, broadening our knowledge and encompassing new questions, the conference will also emphasize the use of experimental approaches to test hypotheses decisively. As in the past, this Conference provides young scientists and graduate students opportunities to present their work in poster format and exchange ideas with leading investigators from a broad spectrum of disciplines. This meeting is never dull: some of the most significant and contentious issues in biology have been thrashed out here. The 2009 meeting will be no exception.

Genetic toxicology represents the study of genetic damage, the agents that induce such damage, and the biological mechanisms that respond to the consequences of genetic damage. Genotoxic agents are abundant in the environment and are often linked to human activity. The effects these agents on humans include many of the common diseases. Because of the importance of this field and the rapid progress during the last decade, many disciplines in the public and private sectors are involved (e.g., structural, molecular and cellular biology, genetics, biochemistry, epidemiology, toxicology, adverse drug reactions, toxicogenomics and hazard assessment). Thus, individual scientists in both industry and academia can use this conference as a forum to exchange ideas and discuss cross-disciplinary collaborations and research opportunities.

The 2009 Gordon Research Conference on Genetic Toxicology will bring together a broad spectrum of scientists with the aim of disseminating current advances and stimulating debate to address future developments. Genetic toxicology ranges from basic research to applied research, including the transfer of knowledge for policy implications. Thus, the bringing together of basic and applied scientists from academia, industry and government is essential to drive this important field forward in an optimal manner. Participants will include those at the cutting edge of basic research and those driving technology developments within applied areas. In keeping with the ethos of Gordon Research Conferences, while information will be disseminated by speaker and poster presentations, vigorous discussion will be the key focus. Designated discussion leaders, recognized for their skills in each particular area, will guide the audience-based discussions. Special consideration will be given to strategies to improve interactions of junior and senior scientists during the conference.

The 2009 Gordon Research Conference on Genetic Toxicology will take place August 9-14, 2009 at Colby-Sawyer College, New London, New Hampshire, USA. Approximately 150 scientists will attend.

Cancer is fundamentally a disease of abnormal gene/pathway function caused by specific alterations in the genome. The nature of these alterations – genetic and epigenetic, and the mechanisms that lead to such genomic changes is the focus of this GRC series. The field has seen a dramatic shift in the past decade from a pure genetic explanation for cancer to a mixed genetic/epigenetic explanation that has important implications for understanding disease causation, deciphering the pathways affected and devising new strategies for prevention and treatment of cancer. Sessions in the meeting will be devoted to recent developments with particular emphasis on whole genome approaches that are revolutionizing the field. Sessions will discuss genomic alterations (mutations, chromosomal changes) and epigenomic alterations (DNA methylation, histone modifications) in cancer in humans as well as in-vitro or animal models. Interactions between genetic and epigenetic changes will also be highlighted, as will clinical implications of the findings, with emphasis on drugs that target genetic or epigenetic changes.

The 2009 Gordon Conference on Muscle: Excitation/Contraction Coupling will present results of the latest research on the cellular organelles and molecular complexes that link excitation to contraction of muscle. The conference will emphasize the multi-disciplinary approaches used, including molecular structure, electron microscopy, biochemistry, molecular genetics, cell physiology, and animal studies. Speakers will address molecular interactions controlling the gating of ryanodine receptors, global and microsocopic calcium movements across the plasma membrane and from the endo-sarcoplasmic reticulum into the myoplasm, mitochondrial calcium signaling, and the regulation of calcium signaling during muscle activity and fatigue. Speakers will also discuss roles of calcium in membrane damage and repair, and in muscle development, adaptation, aging and disease. This conference will bring together investigators who are recognized world leaders in their fields, as well as students and junior scientists who will have the opportunity to present their work in poster sessions and to interact with these world leaders. The organizing committee will select several poster presenters to give short talks during the formal sessions. Overall, this Conference will provide a collegial atmosphere for the exchange of ideas during scheduled discussion periods and during informal get-togethers in the afternoons and evenings and foster new research directions and scientific collaborations.

The 2009 Gordon Conference on Chronobiology will present cutting-edge research on the molecular, cellular and systems aspects of circadian biology. The Conference will feature a wide range of topics, such as the dynamics of transcription and cell cycles, molecular mechanisms of clocks, genetics of human clocks, metabolism and clocks, novel functions for clock genes, model organism clocks, entrainment mechanisms, and the neurobiology of the suprachiasmatic nucleus. Invited speakers represent a variety of scientific disciplines, including biochemistry, molecular genetics, genetics, genomics, structural biology, cell biology, imaging, modeling, sleep medicine, neuroscience, behavior and human biology. The Conference will bring together a collection of investigators who are at the forefront of their field, and will provide opportunities for junior scientists and graduate students to present their work in poster format and exchange ideas with leaders in the field. Some poster presenters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented.

The CAG Triplet Repeat Disorders are a group of largely untreatable inherited neurological disorders which result from an expansion in a CAG trinucleotide repeat in the mutant genes. This group of diseases includes Huntington’s disease, spinal and bulbar muscular atrophy, and several of the spinocerebellar ataxias. In each case, the CAG repeat lies within the coding region of a gene and results in an abnormally long polyglutamine tract within the mutant protein. Marked similarities in the underlying genetics and neuropathology suggest common pathologic mechanisms among these disorders. The 2009 Gordon Conference on CAG Triplet Repeat Disorders and the associated Graduate Research Seminar (GRS) will gather together young investigators and established senior scientists to deliver provoking lectures on the cutting-edge of science. Topics will include a clinical and pathological overview, circuitry malfunction, synaptic pathology, novel neuroimaging techniques, repeat instability, RNA gain of function, bidirectional expression, new animal models, and genetic modifiers. Strong emphasis is placed on training and mentoring of young scientists, and time will be devoted to career issues. All participants will be required to present posters. Some poster presenters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented. Priority will be given to women, minorities and persons with disabilities when selecting participants.

Human genetics is in the midst of a revolution. With the complete sequencing of several human genomes and the exponential decrease in the costs of whole-genome sequencing, the promise of individual genome sequencing is imminent. Despite these advances, a large fraction of the genetic variation underling human disease and phenotypic variation remains unexplained. This Gordon Conference will bring together international leaders in the field of human genetics and genomics to discuss what has been learned and where the field is heading. Speakers will contrast the relative contribution of rare and common variation to disease, summarize the major advances in our understanding of the organization and function of our genome, discuss new insights into human genetic diversity and highlight the application of new sequencing technologies. The conference should be of interest to researchers and students in the fields of human and medical genetics as well as those interested in genome biology in the post-genomic era. It will also provide a venue for rapid exposure to the "state of the art" for investigators contemplating studies, or the utilization of tools, in this field.

Topics:

• The Genetic Basis of Disease
• Human Genetic Diversity and Adaptation
• Human Chromosome Biology and Dynamics
• Genome Technology
• Human Genome Dynamics
• Models and Therapeutics of Human Genetic Disease

Apoptosis plays a key role in the morphogenesis during development and in the maintenance of tissue homeostasis in adults. Its mechanisms have been intensely studied, and early stages of apoptosis are about to be fully understood. The fate of later stage apoptotic cells remain to be characterized.

The 2009 GRC on Apoptotic Cell Recognition & Clearance will present up-to-date research on the molecular, cellular and immunological aspects of the clearance of dying cells. It will feature a wide range of topics, like tissue homeostasis, stress-response, inflammation, and immunology and will focus on the importance of the clearance of apoptotic cells when investigating development, morphogenesis, tolerance, autoimmunity, and immune privilege. The invited speakers represent a variety of scientific disciplines, including developmental research, genetics, signaling, immunology, medicine, and cell biology. Data on various multicellular organisms from worm to human will be presented. The conference will provide a platform for investigators who are at the forefront of their field, and will welcome junior scientists and graduate students presenting their work in poster format to exchange ideas and hypotheses with leaders in the field. We encourage all poster presenters to give short on-spot talks during extended poster sessions. Some of the posters will also be selected for talks in the main auditory.

The conference provides a forum for:

• sharing the latest knowledge on fundamental issues how phagocytic cells recognize apoptotic cells and how pathways for the induction of phagocytosis are activated;
• clarifying implications of apoptotic cell clearance in morphogenesis and homeostasis;
• understanding recent progress in clinical applications;
• evaluating topics outside the main issues of this conference;
• exchanging questions/answers, opinions, ideas and data from ongoing experiments to create collaborations.

The collegial atmosphere of this conference, with opportunities for informal gatherings, promotes cross-disciplinary collaborations in the various research areas represented.

The overall theme of the 2009 Bones and Teeth Gordon Research Conference will be �Stem cells and regulation of bone modeling and regeneration’ covering topics selected based on their emerging importance in skeletal biology (defined as bone, cartilage, teeth and associated soft tissue). The Program will include sessions on Cartilage biology and disease, Skeletal development and remodeling, Dental development and disease, Genetics and bone diseases, Osteoblastic and hematopoietic stem cells, Osteoimmunology, Bone repair and regeneration, and Therapeutic anabolic targets in bone diseases. These sessions will be chaired by leaders in the field who will foster discussion among invited speakers and students and post-docs in a collegiate atmosphere. Through presentations and extensive discussion, new understanding of skeletal metabolism in health and disease will lead to continued development of new therapeutic strategies for common and rare bone diseases. A new development for the Bones and Teeth GRC this year is a Graduate Research Seminar. This will be a two-day symposium beginning on July 11, the day before the start of the GRC to which post-docs and graduate students from the bone field will be invited to present their best unpublished research in the form of oral presentations and posters. The theme of the Seminar will be Advances in Understanding of Bone and Cartilage Development. Participants in the Seminar will be expected to remain for the whole of the GRC where they will have the opportunity to present their research and discuss their findings with senior members of the bone research community.

The 2009 Gordon Conference on Cellulases, Cellulosomes and other Carbohydrate Modifying Enzymes will present cutting-edge research on the enzymatic degradation of cellulose and other plant cell wall polysaccharides. The Conference will feature a wide range of topics that includes the enzymology of plant structural degradation, regulation of the degradative apparatus, the mechanism of protein complex assembly, the genomics of cell wall degrading organisms, the structure of the substrate and the industrial application of the process particularly within the biofuel arena. Indeed the deployment of plant cell wall degrading enzymes in biofuel processes will be an important feature of the meeting. It should be emphasized that the 2009 Conference will be expanded to include, in addition to cellulase research, recent advances in other plant cell wall degrading enzymes, and contributions from people working on hemicellulases and pectinases will be particularly welcome. Invited speakers represent a variety of scientific disciplines, including biochemistry, structural biology, genetics and cell biology. The interplay between fundamental research and its industrial exploitation is a particularly important aspect of the meeting, reflecting the appointment of the chair and vice-chair from academia and industry, respectively. The meeting will provide opportunities for junior scientists and graduate students to present their work in poster format and exchange ideas with more established figures in the field. Indeed, some poster presenters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented. The Conference is likely to be heavily subscribed so we would recommend that you submit your application/abstract to the GRC website as soon as possible.

The 2009 Gordon Research Conference on Molecular and Cellular Biology of Lipids will bring together leading scientists in the field to present and discuss their research on lipid metabolism. The meeting will be held July 19-24 at the Waterville Valley Resort in Waterville Valley, New Hampshire and will continue a near 50-year tradition emphasizing the roles of lipids in health and disease. The meeting will include nine lecture sessions and four afternoon poster sessions. The lecture sessions will cover a broad range of topics, including regulation of lipid metabolism by nuclear receptors, bacterial lipid synthesis and catabolism, genetics of lipid metabolism, enzymes of lipid metabolism, trafficking of proteins and lipids, protein structure in the lipid bilayer, mammalian lipid metabolism, and lipid localization and membrane structure. The 37 speakers in these sessions will report research using a wide range of in vivo and in vitro biological systems and employing multiple experimental approaches. Lectures at this meeting are well known to stimulate lively discussions among attendees who in this case will include biochemists, structural biologists, cell biologists, and geneticists. Scientists who attend this conference derive synergistic benefit from the high quality of the speakers, the diverse experimental backgrounds of the participants, and the resplendent natural surroundings of the White Mountain National Forrest. These benefits in turn drive future advances in lipid metabolism. Participants and speakers will reflect international excellence in this medically-relevant area of science with an ideal balance between youth and wisdom.

As research in the plant sciences begins to adopt systems biology approaches, exciting opportunities continue to emerge to redesign and engineer the biochemical and molecular networks responsible for agronomic traits, increased nutritional value, and for the generation of alternative, renewal fuels, and commercial products. This is the third Plant Metabolic Engineering GRC to integrate the most recent advances in genetics and genomics with biochemical, metabolite and gene expression analyses. Fundamental topics covered include i) understanding how biosynthetic pathways are assembled to facilitate substrate competition and channeling, ii) how enzymes and biochemical pathways evolve, and iii) exploring opportunities for the manipulation of biochemical transformations towards novel substrate specificities, regio- and stereochemical transformations, and models to explain the localization, structure, and assembly of biosynthetic metabolons in native and nonnative environments. This GRC provides the ideal environment to present and discuss recent discoveries and novel approaches for metabolic profiling and flux analysis, the mathematical modeling of metabolism and conceptualization of rational metabolic engineering strategies. The Conference brings together multidisciplinary investigators from around the world who are at the forefront of their field, and will provide opportunities for junior scientists and graduate students to present their work and exchange ideas with leaders in the field. Some short talks will be selected from the abstracts submitted as part of the registration process and from poster presentations. The collegial atmosphere of this Conference, with programmed discussion sessions and opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to brainstorm and promotes cross-disciplinary collaborations in the various research areas represented.

Triplet repeats occur naturally throughout the genome. Triplet repeat disorders result when triplet repeat numbers expand above some critical threshold. How expanded triplet repeats result in a variety of human genetic disorders is a growing field and spans many interdisciplinary fields of study including genetics, pharmacology, biochemistry, cell biology, neurology, and psychiatry.

The goal of the Graduate Research Seminar (GRS) in triplet repeat disorders is to provide opportunities for graduate students and postdoctoral fellows to showcase their work in a collaborative atmosphere. The aim of this event is to provide a forum for young scientists to discuss and to present their innovative, unpublished findings in a supportive environment, to spur stimulating discussions led by recognized leaders in triplet repeat disorders, and to foster the growth of new ideas and form new interdisciplinary collaborations for future research.

Individuals interested in the field of triplet repeat disorders are encouraged to apply and to submit an abstract. Participants are expected to either present a poster or deliver an oral presentation. Poster presenters and speakers will be selected from the abstracts. Application to the "Triplet Repeat Disorders" Gordon Research Conference (GRC) is separate from the GRS, and we strongly encourage GRS attendees to also apply for the GRC. Travel support and scholarships are available. However, travel funding is only available to GRS participants taking part in both meetings and early application is encouraged, as space is limited.

Application Deadline: All applicants for registration to the Graduate Research Seminar (GRS) on Triplet Repeat Disorders must submit an abstract for a poster presentation by April 1st, 2009. Speakers will be invited from the pool of poster applicants. Please limit abstracts to 250 words. The GRS is a two-day meeting held prior to the GRC, and all student and postdoctoral applicants for registration to the GRS-Triplet Repeat Disorders are highly encouraged to apply for registration to the Gordon Research Conference (GRC) in Triplet Repeat Disorders. Applicants may present the same poster and abstract at both the GRS and the GRC. We hope to cover partial or all registration costs for GRS participants. Additional awards for travel may be available for minority applicants. For more information please contact the GRS and GRC chairs, Mary Heng and Jang-Ho Cha.

Quantitative genetics aims to link phenotypic variation to its underlying genetic basis in order to better understand and predict genetic composition and long term change within natural, agricultural, and human populations. Traditionally built upon statistical abstractions of genetic effects, the field is now rapidly advancing by making use of recent developments in genetic technology to reveal explicit links between genes and complex phenotypes, and therefore serves as a focal point bringing together many emerging areas of genetics, genomics, physiology, statistics and computational biology. This synthesis is already having a large impact on the areas of evolutionary biology, plant and animal breeding, and the analysis of human disease.

The strength of the Gordon Conference on "Quantitative Genetics and Genomics" has historically been the bringing together of leaders in each of these fields to explore areas of shared interest and cutting edge developments with the potential to affect all researchers within the broad field of quantitative genetics. Building upon the tremendous success of our conferences in recent years, we will again focus on many cutting-edge developments in the field, including whole genomic approaches to understanding genetic variation, genome architecture and regulation, systems biology, non-traditional forms of inheritance, and statistical genetics.

See you in Galveston!
Daniel and Peter

Contributors: We are extremely grateful to the many companies and organizations that have made financial contributions in support of this conference. Their generous contributions have helped us provide a large number of scholarships to help graduate students and postdocs attend the meeting (to apply please contact us at grc2009@gmail.com), as well as help us pay for some of the various costs associated with speakers and social events. The contributors to date include are listed below. We are still accepting contributions to this meeting!

Plant metabolic engineering is a rapidly developing field that demands multidisciplinary approaches in plant genetics, genomics, biochemistry, physiology, chemistry and cell biology. It is a field with great socio-economic ramifications, as it aims to positively influence agriculture, the environment, and human health and wellness. The Gordon-Kenan Graduate Research Seminar (GRS) in Plant Metabolic Engineering was initiated in 2007 to provide a unique opportunity for future research leaders to share their work in this field. The 2009 meeting will continue this tradition of providing an environment conducive to stimulating scientific discussion and constructive, yet critical assessment of each other’s work, without the undue influence often associated with the presence of senior investigators. The GRS immediately precedes the Plant Metabolic Engineering Gordon Research Conference and is designed specifically for and by graduate students and post-doctoral scholars. Graduate students and post-docs working in plant metabolic engineering and all related disciplines around the world are enthusiastically encouraged to apply to attend the 2009 GRS and GRC in Plant Metabolic Engineering. Travel and registration awards for US and international participants are anticipated.

The Gordon Vascular Biology conferences have been inaugurated 21 years ago and have become a highly anticipated event in the field. The study of vascular biology continues to proceed at a remarkable pace. Many new molecules relevant to vascular development and angiogenesis have been discovered in the last 2 years. Importantly, advances in vascular biology have contributed to the development of many new therapies and preventive strategies.

The 2009 conference will cover a variety of traditional as well as novel vascular biology topics including development of the vascular system, cell-cell and cell-matrix communications, proteoglycans and the vasculature, intracellular signaling, vascular permeability and maintenance, and hypoxia and metabolic regulation. The meeting will bring together speakers, discussants and participants that represent a wide range of disciplines, approaches and systems. The small size of the conference and the informal atmosphere will facilitate discussion and interactions. The theme of the conference makes it highly relevant to scientists working in various disciplines such as developmental, cell and molecular biology, genetics, cell signaling, and immunology among others, as well physicians and scientists seeking better understanding and treatment of various disease processes including cancer, cardiovascular disease and inflammation.