SUPER-AGAROSE

Size-Select and Concentrate your DNA or RNA right in your Gel!

Recovering intact nucleic acids from a gel is simplified with our special formulation of Super-Agarose.

Once you have run your gel, cut a small trough in front of your band(s) of interest, fill it in with our ready made Super-Agarose, and electrophorese in the nucleic acid. Super-Agarose's formulation decreases the DNA's electrophoretic mobility to near zero; thus messy broad bands, and even multiple bands, "pile-up" and are compressed into a minimum volume of agarose (fig. 1). That means you get all of your nucleic acid in a few microliters of high-quality, low-melting temperature gel, rather than in a few hundred microliters. Plus there is no loss or shearing of high molecular weight molecules since they remain in the gel.

Use Super-Agarose to obtain preparative amounts of DNA or RNA from minor bands, or of size-selected genomic DNA (fig. 2). Run your gel as usual, loading several adjacent lanes with the same sample of interest; cut out the appropriate band across those lanes; cut another perpendicular hole, slightly longer than your band slice, elsewhere in the gel (or in a new gel). Place your band slice in the new hole and fill the extra space (toward the anode) with Super-Agarose (fig.2). Your nucleic acid will run until it becomes highly concentrated within the Super-Agarose: ready for your demanding application! (fig.2) Virtually all enzymatic reactions can be performed "in gelo" or you can extract if you wish.

Every batch of Super-Agarose is tested and guaranteed to be free of nuclease, protease, and "enzyme" inhibitors.

Figure1.
a) DNA from .8-2kb concentrated on Super-Agarose
b) Same sample, no Super-Agarose
 

Figure 2
Protocol for preparative "in gelo" concentration of DNA or RNA (see text for description)
 

2 ml: $ 11.00† (10-40 gels)
12ml: $ 49.00† (60-240 gels)
25 ml: $ 59.00† (120-480 gels)

Our little Hand-held UV Lamp Is Big on Illumination

Not a new concept, but an AMAZING price!  These hand-held UV lights illuminate ethidium bromide stained DNA superbly (as good or better than the common models costing over 5 times as much).

Our little hand-held UV	Traditional hand-held UV

Hand-held UV Lamp: $43.00†

GELS 3.0

• Restriction fragment mapping
• Peptide mapping
• Protein, RNA, and DNA fragment identification and sizing
• No need to put a ruler in your gel pictures

Automatic Marker Entry!
GELS 3.0 is an amazing time saving innovation - simply tape a photo or autoradiogram of your gel to your Macintosh screen and the program prompts you to click in your marker bands using the mouse and cursor! Use the Markers menu to select any of the commonly used DNA and protein marker sets and the program will automatically prompt you to enter each band in the set by clicking on them, or you can select Other Markers and enter your own

Band Input
Click on your experimental bands lane by lane. Each time a band is clicked, its Molecular Weight is calculated automatically, based on the markers you entered, and instantly displayed, along with the total Molecular weight of all bands in that lane. Simultaneously, GELS 3.0 displays a mock gel on the screen.

Data Output
Select Preview Bands and GELS 3.0 scales your data to produce a clear full-screen image of the mock gel. Select Preview Sizes and each band in the mock gel is replaced by the text of its Molecular Weight! Print Bands and Print Sizes optimize the output for your printer and produce beautiful data for your notebook or group meeting discussions.

Merging Data
The merge Data command lets you compare experiments performed on separate gels, with separate size markers, or a small photo of a gel with a life-sized autoradiogram of that same gel, side by side, so that bands can be identified easily.

Editing Data
Full Cut-and-Paste editing of the bands and lanes in the mock gel is supported so that you can experiment with possible doublets and faint bands until a logical consistent restriction map emerges.

If nucleic acids are not your favorite molecules, GELS 3.0 is just as useful for dealing with proteins, carbohydrates, or any other molecules you run through a sizing gel. 50 lanes of information can be stored at once (with 50 bands per lane). It is difficult to list all the features of Gels 3.0, but easy to use them. Full on-line help and documentation are provided (though seldom needed!)

$ 50.00†

OLIGO-DESIGNER 1.0

Design Oligo-Nucleotide Probes in about 1 minute!

Oligo-Designer 1.0 maximizes your chances of cloning genes by protein sequence homology by helping you choose probes with the lowest degeneracy. It takes your protein sequence (which you can type in, or cut and paste from anywhere) and Reverse-Translates it for you. The key is in the graphic form of the output: the degenerate nucleotide code "hangs down" from the amino-acid sequence. This makes finding the least ambiguous sequences quick and easy - just look for indentations (contiguous areas where the least is hanging down) and you have your probe sequences, ready to synthesize and clone your gene!!! It's an almost instantaneous process.

$ 30.00†