A microforge is a versatile instrument designed specifically for the
fabrication and processing of glass micropipettes and other related
microtools for use in patch pipette tip polishing, tip size reduction
(for holding pippettes), contact stretching (large tip sharpening),
tip bending, tip sealing, in vitro fertilization (IVF),
intracytoplasmic sperm injection (ICSI), and pipette production in a
variety of other pipette configurations.
Narishige microforges use advanced design and manufacturing to provide
an ultra-fine finish to microelectrode tips for patch clamp
applications by eliminating imperfections on the microelectrode
surface which would otherwise damage delicate membranes when making
tight patch seals. Their design makes locating and polishing the
pipette under magnification extremely easy.
Fire polishing of patch pipettes is the single most important factor
for forming a stable giga-seal in patch clamp recording ? even more
important than the type of glass capillary used. Narishige microforges
provide superb seals with a wide variety of cells. Both whole cell
patch pipettes and single channel patch pipettes can be conveniently
polished to the highest quality and reproducibility.
Holding pipettes generally have large blunt tips with a small opening,
which are used to hold a free-floating cell in place prior to
microinjection by applying a low-pressure suction to the rear of the
pipette. Production of holding pipettes involves squaring it off,
large bore flame polishing, and tip reducing.
Often a beveled large bore pipette is not sharp enough to penetrate a
cell without damaging the area around the pipette. A sharp point can
be formed on the beveled tip to assist the penetration of the cell;
this process is referred to as contact stretching.