MINJ-1000


microINJECTOR™ System Complete w/ Inverted Microscope, manipulator

MINJ-1000:  $11,891.89 - Special Order
Tritech Research sells complete microinjection systems that integrate our famous microINJECTOR™, Pulse Length Control Module (PCM), and needle holder with top name brand inverted microscopes and micromanipulation equipment. We take advantage of our status as a fully authorized Narishige dealer and manufacturer of custom OEM solutions for Olympus and other microscope systems to get you great prices and the added convenience of receiving a fully integrated microinjection system from a single source.

     Other features include our custom GlideStage®, which provides a fine level of manual stage manipulation at a fraction of the cost of motorized stage manipulators. When the stage is in use, you can employ a low-cost mechanical joystick-type micromanipulator, to save thousands of dollars over other systems.


Microscope Features:
  • Small Foot Print.
  • Excellent Quality Optics.
  • Includes our custom-made Hoffman Modulation Contrast (HMC) optics for DIC-like images at a significantly reduced price.
  • Trinocular head for photo and video documentation.
  • Available with a fluorescent system for detection of GFP, DAPI, Rhodamine, etc.


Some researchers are curious whether a low-cost dedicated injection station like the MINJ-1000 is going to be a compromise in terms of quality, ease-of-use, or optics. The answer is - no. Great optics are as important to us as they are to you. Just as there can be a "good" DIC image, when the right Wallaston prism is combined with the right numerical aperture objective and the correct polarization, and a poor DIC image when these items are not optimized, we design and choose our HMC optics to work together to get you great images. Don't let a bad experience with a local microscope rep who doesn't know what he is doing, or understand how to optimize a system for your microinjection needs prejudice you.

Here are links to some QuickTime movies showing oocytes and the syncitial gonads of some C. elegans worms under our MINJ-1000 scope. We think you will be impressed with how clear and easy to inject these structures are. Click on these links to watch the QuickTime Movies (it will take a while for them to load completely, and then they will play.) To watch one again, click "Reload" or hit the play button if it is visible:

gonad04.mov (6.2MB)
gonad05.mov (7.3MB)
gonad06.mov (4.0MB, lower res)

And, here are a few still images... Click them to see an enlarged view:

Gonad 14
Gonad 15
Gonad 16

COMPARISON OF HOFFMAN AND DIC VIEWS
OF THE SAME C. ELEGANS WORM


Worm HMC vs DIC Comparison
HMC
Worm DIC Diagram
DIC













FAQ's

Q: What are the dimensions of this system, in terms of being compact and fitting on my injection table?
A: The microscope itself is 21" (54cm) deep from the eyepieces to the back x 9" (23cm) wide; it is 21" (54cm) tall. Our standard joystick-type micromanipulator mounts on the side of stage and adds about 4" (10cm) of width there.

Q: Which exact components are included in the standard MINJ-1000?
A: The MINJ-1000 includes the inverted microscope, the Narishige MN-151 joystick micromanipulator, our Analog foot-pedal controlled microINJECTOR MINJ-1, pulse-length control module MINJ-2, brass straight-arm quick-change needle holder MINJ-4, and custom-made microinjection GlideStage MINJ-GS. The standard optics for the worm version of the MINJ-1000 includes 2 Olympus objectives: a 4x for finding the worms and a customized 40x short working distance HMC objective for optically sectioning the worm to find the z-plane with the gonad of interest.
Some people choose to upgrade to our digital microINJECTOR, a hydraulic micromanipulator, or to add our low-cost anti-vibration bench plate or a camera, or fluorescence capabilities. These are all extra options for additional cost.

Q: What are some other items needed to start injecting procedures immediately using the MINJ-1000 system?
A: 1) 50mm x 24mm cover slips
2) agar for agar pads
3) something like an oven to bake the agar pads
4) halocarbon oil, or at least mineral oil, to cover the agar pads and protect the worms from total desiccation
5) a good non-vibrating table, or our MINJ-AVP
6) a nearby electric outlet for the scope and the MINJ-2
7) a nearby dissecting scope to quickly mount and recover the worms, such as our SMT1 stereo microscope.

Q: How can I adjust the camera/eye piece so that camera and eye piece are both in focus?
A: Since the camera should be parfocal with the eyepieces on the MINJ-1000 by the nature of c-mount optics, perhaps one too many (or possibly one too few) of the threaded adapter rings got moved along with the camera. Please try removing or adding a ring in between the camera and the c-mount for the MINJ-1000 and see if that makes it parfocal. One way that you can tell the needed distance is to remove all of the rings and hold the camera above the c-mount adapter by hand to see how high it needs to be for the image to be in focus. Also, please note the the eyepieces have a diopter adjustment, and parfocality should be near the "0" point on the eyepieces.

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.