EG-T2D


Micropipette Grinder w/ Digital Microscope

EG-T2D:  $5,940.54 † - In stock

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Medium Grit size

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EG-T2D Micropipette Beveler / Precision Microgrinder

Precision Beveling for Microinjection Needles, Patch Clamp Pipettes, and Microtools

The EG-T2D Microgrinder System from Tritech Research is a next-generation micropipette beveler and precision microgrinder designed for beveling pulled glass microinjection needles, opening patch clamp pipettes, and shaping fine glass or metal microtools under direct microscopic observation.

The EG-T2D was developed as a modern alternative to older-style micropipette bevelers and microgrinders from companies such as Narishige, Sutter Instrument, and World Precision Instruments. Researchers searching for systems comparable to the Narishige EG-45, EG-400 / EG-401 / EG-402, Sutter BV-10, or other traditional microelectrode beveling systems will find that the EG-T2D introduces substantial improvements in visibility, positioning control, abrasive replacement, and operational flexibility.

Unlike legacy microgrinders that use fixed whetstones, limited positioning control, and cumbersome conventional microscopes, the EG-T2D combines precision X-Z micromanipulator positioning, an integrated digital microscope with LCD display, interchangeable magnetic abrasive disks, adjustable bevel geometry, wet grinding capability, and a compact laboratory footprint.

Applications for Modern Microinjection / Electrophysiology Labs
  • Microinjection needle beveling
  • Patch clamp pipette polishing
  • Making intracellular recording micropipettes
  • Oocyte injection needles for IVF and transgenic production
  • Cell / embryo manipulation pipettes
  • Fine polishing of metal probes and microtool fabrication
  • Glass needle opening



Precision Micropipette Beveling w/ Direct Visual Control


The EG-T2D allows the user to position a glass micropipette or microtool directly above a rotating abrasive disk while observing the grinding process in real time using the integrated long-working-distance microscope.

The operator can precisely adjust:
  • X position using the micromanipulator X-axis
  • Z position using the micromanipulator Z-axis
  • Y position by rotating the manipulator on its mounting pole
  • Grinding angle by rotating the pipette holder mounting bracket

This geometry provides significantly greater control compared with traditional single-axis micropipette positioners. Researchers can optimize bevel angle, contact pressure, grinding location, magnification, focus geometry, and tip opening characteristics for a wide variety of experimental applications.

Integrated Digital Microscope with LCD Display


The integrated long-working-distance digital microscope eliminates the need for a separate, difficult-to-position dissecting microscope, or the cumbersome, costly optical microscopes integrated with old-fashioned units.

Features
  • Integrated LCD screen
  • Digital zoom capability
  • Long working distance optics
  • Rechargeable cordless operation
  • USB-powered continuous operation
  • Adjustable viewing geometry
  • Fine aiming controls

The optical geometry was specifically designed for micropipette beveling and microtool grinding applications. Maximum magnification is achieved when the pipette tip is positioned approximately 5-10 mm from the front edge of the abrasive disk while still maintaining sharp focus.

Interchangeable Magnetic Abrasive Disks


Unlike older whetstone-based microgrinders that require periodic resurfacing and difficult, costly replacement, the EG-T2D uses lower-cost, interchangeable, magnetically-backed abrasive disks that mount to its stainless steel platen.

Benefits
  • Fast tool-free disk replacement
  • Lower consumable cost
  • No stone redressing required
  • Multiple abrasive grades available
  • Easier maintenance

Each EG-T2D system includes one abrasive disk of your choice:
  • 3 µm abrasive disk (standard for fine bevels and polishing)
  • 6 µm abrasive disk (more aggressive)
  • 9 µm abrasive disk (for aggressive grinding)

Additional replacement disks are available for only $65 each. The magnetic mounting system allows the user to simply peel off the old disk and place a new one onto the stainless steel support platen in seconds. Some laboratories use multiple grit sizes sequentially for shaping and fine polishing.

Optimized Wet Grinding for Cleaner Micropipette Tips


The EG-T2D uses wet grinding to improve bevel quality and reduce debris accumulation.

Applying distilled water to the abrasive disk:
  • Produces a polishing slurry
  • Reduces electrostatic particle attraction
  • Improves bevel smoothness
  • Extends abrasive disk life

An integrated drain port allows wastewater collection when larger amounts of water are used.
Unique feature: Positive pressure can be applied through the special pipette holder during grinding to prevent water from entering ultrafine pipette tips by capillary action.

The system accommodates a broad range of bevel geometries and grinding conditions, making it useful for both routine laboratory workflows and advanced custom microtool development.

Why Researchers Upgrade from Older Microgrinders

Researchers familiar with older systems such as the Narishige EG-401, EG-402, or similar microelectrode bevelers often encounter:
  • Expensive fixed grinding stones
  • Limited positioning control
  • Difficult abrasive replacement
  • Lack of integrated imaging
  • Limited grit options
  • Complex water delivery systems

The EG-T2D addresses these limitations with:
  • Precision X-Z control
  • Integrated LCD microscope
  • Magnetically mounted abrasive disks
  • Multiple grit options
  • Simplified wet grinding
  • Faster setup and maintenance
  • Lower consumable cost


Here is a video that shows the EG-T2D beveling a needle:


To watch the video larger, Click the full-screen arrows.
To watch in a separate window with captions, Click on "Watch on Youtube"


FAQ's

Q: How do I open the tip of a pre-pulled needle without compromising the sharpness of the needle or using razor blades to cut the tip?
A: There are three methods. One of the most straightforward to to bevel the needle with microgrinder like the EG-T2D. The result is a hypodermic-style tip that is open, but has an extremely sharp leading edge. Other methods include using hydrofluoric acid to etch the needle tip or a microforge, like the MF3 to very precisely break the tip using thermal shock.

Q: How easily can I change the abrasive disk?
A: The abrasive disks are magnetically backed and require no tools for replacement. Simply:
  • Lift the old disk from the edge
  • Remove it
  • Center the new disk
  • Lower it onto the stainless steel platen

Q: What is the purpose of wetting the grinding disc and why should I bother?
A: There are several reasons why it's a good idea to perform microgrinding with a wet grinding disc. Microscopic diamond particles dislodge from the disc and, in the presence of water, these form a slurry that contributes to the grinding and polishing process. This allows for faster grinding and gives a smoother result compared with using the disc dry. The grinding process generates static electricity, and this will cause debris to adhere to the needle tip; water drastically reduces this. Finally, if the disc is used wet, it will last many times longer than if it's used dry. Note: use double-distilled de-ionized water or equivalent to prevent the build up of minerals as the water evaporates.

Q: What types of glass and objects are compatible with the EG-T2D and why should I choose one type of glass over another?
A: The system is commonly used with:
  • Borosilicate glass
  • Aluminosilicate glass
  • Quartz glass (fused silica)
  • Fine metal probes
  • Microtools

Aluminosilicate and quartz glass typically flex less than borosilicate glass during grinding and thus can produce sharper bevels by not bowing as much under pressure.


Specification
Accessories included X-Z micromanipulator with angle plate
Dedicated Pipette Holder w/ pressure control
Water application brush
Power Adapter
USB cable
Movement range Z-axis fine movement: 15 mm
X-axis fine movement: 15 mm
Magnifications ~5x - ~200x
Glass capillary O.D. 0.5mm - 2.0mm
Working voltage AC100V - AC240V, 50/60Hz
Power consumption 24W
Moter speed Approx. 100 - 1,200 rpm
Dimensions/Weight W180 x D180 x H230mm, 1 kg

† These are our list prices. If you are paying with an Institutional Purchase Order or by check, you qualify for a 7.5% discount. Click here to change your payment method and see the lower prices.