Yeast Protocols
Click on the links below to jump to a particular section.
Yeast Culture and Storage
Yeast Genetics
Transformation
Two-Hybrid System
Saccharomyces Genome Database Links
Yeast Culture and Storage
Media
(Bowtell Lab)
Media
(Harvard medical school)
Streaking yeast stocks
(Donis-Keller lab)
Long term storage of yeast stocks
(Donis-Keller lab)
Media & Solutions & Stocks
(Donis-Keller lab)
Sporulation & Dissection
(Hahn Lab)
Freezedown guidelines
(
Saccharomyces
Genome Deletion Project)
Liquid sporulation
(Herskowitz Lab)
Old yeast cell sorting protocol
(Sinclair Lab)
YPD filled microtiter plates
(
Saccharomyces
Genome Deletion Project)
Candida methods
(University of Minnesota)
Yeast protocols handbook
(CLONTECH, pdf)
Preparation of yeast cells for immuno-electron microscopy
(BioVisa.net, pdf)
IF with methanol/acetone dehydration
(Botstein Lab)
Cell cycle by flow cytometry
(Salk Institute)
FACS analysis
(Sinclair Lab)
Extraction and purification of ascorbic acid analogs in biological samples using HPLC
(BRIC)
Preparation of gel samples for pulsed-field gel electrophoresis
(BRIC)
Purification of D-arabinono-1,4-lactone oxidase from the mitochondrial fraction
(BRIC)
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Yeast Genetics
Pheromone halo assay
(Dohlman Lab)
UV mutagenesis
(Corbett Lab)
Replication timing by density transfer
(Fangman / Brewer Lab)
beta-Gal reporter gene assay
(Dohlman Lab)
beta-Gal filter assay
(Dohlman Lab)
beta-Gal filter assay
(Breeden Lab)
b-Gal filter assays
(Herskowitz Lab)
EMS mutagenesis of yeast
(Hahn Lab)
Replication timing by comparative hybridization
(Fangman / Brewer Lab)
Replication timing using transient hemimethylation
(Fangman / Brewer Lab)
X-Gal agarose overlay assay
(Herskowitz Lab)
Procedure for generating ts alleles of NUT2
(Herskowitz Lab)
Yeast Genetics Protocols
(Herskowitz Lab)
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Transformation
Transformation of yeast
(Bowtell Lab)
Modified yeast transformation
(Bowtell Lab)
Isolation of shuttle vectors from yeast and transformation into E. coli
(Bowtell Lab)
Electroporation
(Sinclair Lab)
High efficiency yeast transformation
(Trembath Lab)
DNA transformation into yeast: lithium acetate method
(BRIC)
DNA transformation into yeast: spheroplast method
(BRIC)
Rhodamine-Phalloidin/Calcofluor Staining
(Botstein Lab)
Transformation, RNA miniprep, Genomic DNA miniprep, Chromosomal DNA miniprep, b-GAL filter assay, FACS on Yeast
(Breeden Lab)
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Two-Hybrid System
The yeast two-hybrid system for beginners
(Newman / Collinge Lab)
Two-hybrid system
(Millner Lab)
Yeast Two-Hybrid : State of the Art
(Van Criekinge, Beyaert)
Two-hybrid analysis of genetic regulatory networks
(Finely Lab)
Two-hybrid system
(Finely Lab)
Performing a hunt by interaction mating
(Finely Lab)
GST fusion protein purification from Yeast
(Dohlman Lab)
Two-hybrid system
(Gietz Lab)
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Saccharomyces Genome Database Links
BLAST
FASTA
S. cerevisiae protein structural information
Yeast Maps
Genomic View
Worm-Yeast Protein Comparison
Mammalian Homology to Yeast
Yeast Genome Restriction Analysis
Search
Saccharomyces
GenBank Sequences
Pairwise Chromosome Similarity View
( back to top )
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